Multivitamin–Cisplatin Encapsulated Chitosan Nanoparticles Modulate DDX3X Expression in Cancer Cell Lines

Vitamin supplementation during chemotherapy has often been associated with lower recurrence and mortality rates in cancer patients. We had previously demonstrated that the multivitamin (C, D3, B12)–cisplatin nanoparticle complex—NanoCisVital (NCV)—could alleviate chemotherapy-induced fatigue. Chitosan is frequently used functional nanomaterials to encapsulate drugs, because it biodegradable, biocompatible, non-toxic. The chitosan-based NCVs were prepared, their physicochemical properties, size, stability evaluated before assessing effect on cell lines. mixture packed core, cisplatin loaded at periphery of nanoparticle. This encapsulation facilitates slow sequential release peripheral core combination. By increasing amounts vitamin drug-encapsulated nanoparticles breast cervical lines, viable percentage was calculated. DDX3X promotes proliferation, invasion, metastasis, while Ki-67 active proliferation all types. elevated several types, cells express abnormally. protein a biomarker present throughout stages cycle but undetectable resting state. expression DDX3X Ki-67 genes altered NCV-treated cells. study uses gene as comparative measuring tool for anti-cancer effects vitamins, respectively.